INTENDED USE

This test is intended for the in vitro quantitative detection of Feline Calicivirus antigen (FCV Ag) in feline ocular, nasal, or oral swab specimens, used with a fluorescence immunoassay analyzer. It serves as an aid in the diagnosis of FCV infection, in monitoring viral load during treatment, and in differentiating FCV from other causes of feline upper respiratory tract disease. For professional veterinary use only.

PRINCIPLE

The test employs a quantitative double-antibody sandwich fluorescence immunoassay. FCV antigens in the sample first bind to fluorescent nanoparticle-conjugated anti-FCV antibodies coated on the conjugate pad. The resulting complex migrates by capillary action along the nitrocellulose membrane and is captured at the T line by immobilized anti-FCV monoclonal antibodies. A fluorescence analyzer measures the T-line signal intensity and, using an on-card calibration curve, reports the FCV Ag concentration. The C line confirms the validity and proper flow of the assay.

SPECIMEN

Ocular, nasal, or oral secretion swab. Collect the specimen with a swab, elute it in the provided sample diluent, mix thoroughly, and apply the eluate to the sample well; test promptly after collection and avoid repeated freeze–thaw of stored samples.