INTENDED USE

Qualitative detection of antibodies to Toxoplasma gondii in serum or plasma collected from cats, for veterinary point-of-care use. It aids in assessing exposure to this intracellular protozoan parasite. A positive result indicates prior or current exposure rather than active clinical disease; IgM and IgG interpretation with clinical signs is required, and results must be interpreted together with other diagnostics.

PRINCIPLE

The TOXO Ab Rapid Test is a lateral-flow immunochromatographic assay based on colloidal gold labeling. The test cassette contains a nitrocellulose membrane with a test (T) line pre-coated with capture antigen and a control (C) line. When the processed serum or plasma is added to the sample well, it migrates by capillary action. If anti-Toxoplasma antibodies are present, they bind the gold-conjugated antigen and are captured at the T line, forming a visible colored band. The C line should always appear, confirming the assay ran correctly. Results are read visually by the naked eye within the stated time; no instrument is required.

SPECIMEN

Use serum or plasma collected from cats by venipuncture. For serum, allow clotting and centrifuge; for plasma, use anticoagulated blood. Use fresh samples; avoid hemolysis. If not tested immediately, store at 2–8 °C for a short period or freeze; mix well and bring to room temperature before testing.

REFERENCES

• https://www.vet.cornell.edu/departments-centers-and-institutes/cornell-feline-health-center/health-information/feline-health-topics/toxoplasmosis-cats
• https://www.abcdcatsvets.org/guideline-for-toxoplasma-gondii-infection/?pdf=5268